XXX V] TISSUES OF REPRODUCTION 279 



cover-slip, with simple lens or low objective. If the 

 ovum is present it will at once be recognized. (The 

 whole procedure is best performed under a dissecting 

 microscope.) When one is obtained carefully cover with 

 a cover-slip, inserting a thickish ring of paper in order 

 to avoid pressure, and examine with a high power. 

 Observe 



The thick zona pellucida, with double contour (and 

 radiating striation). 



The granular cell substance (vitellus). 



The transparent germinal vesicle, with its germinal 

 spot. If the follicle be quite ripe these may have 

 disappeared. 



The cells of the cumulus proligerus attached all 

 round the zona pellucida. 



4. Uterus. Transverse section of cornu of uterus 

 of cat (chromic acid and spirit, cut frozen) ; stain with 

 hsematoxylin and (slightly) with eosin. Observe 



The thin external peritoneal coat. 



The muscular coat, consisting broadly of an 

 external longitudinal and an internal circular coat, 

 separated by connective tissue containing largish blood 

 vessels. The size and arrangement of the coats vary 

 in the different parts of the uterus and in different 

 animals. 



In most parts of the uterus the external muscular coat consists of 

 two parts, an outer longitudinal coat, and an inner coat with fibres in 

 various directions. The main circular coat, called above the internal, 

 is taken to correspond with the muscularis mucosaa. 



The mucous membrane, consisting of connective 

 tissue covered with columnar epithelium, and containing 

 long tubular glands, sometimes branched at their ends. 



