284 PRACTICAL HISTOLOGY [XXXV 



layers of cells; the connective tissue layer of the 

 mucous coat containing numerous elastic fibres and 

 blood vessels ; the muscular coat, chiefly longitudinal ; 

 the fibrous coat. 



3. Transverse section of testis and head of epi- 

 didymis, of young pig (formol, cut frozen, Ehrlich- 

 Biondi stain). Note the general features described in 

 the text, 8; the connective tissue and the inter- 

 stitial cells are in relatively greater amount. 



4. Section of prostate gland (dog ; alcohol or 

 mercuric chloride ; eosin and hsematoxylin). Note the 

 irregularly shaped alveoli lined by one or two layers of 

 cubical to columnar cells ; and between the alveoli, the 

 considerable amount of fibrous tissue with elastic fibres 

 and unstriated muscle. 



5. Section of erectile tissue (dog; potassium 

 bichromate, eosin and hsematoxylin). Note the sponge- 

 work of connective tissue containing bands of unstriated 

 muscle, and covered with flat epithelium; the spaces 

 are the venous sinuses. 



NOTES. 



The ovary may be hardened in mercuric chloride, chromic 

 acid and alcohol, or Flemming's fluid, and stained with any of 

 the usual stains. 



The Fallopian tubes, uterus, vagina, vas deferens are perhaps 

 best hardened in potassium bichromate, but alcohol, mercuric 

 chloride, or one of the formol mixtures may be used. The testis 

 may be hardened in mercuric chloride or a formol mixture to 

 show the general features : and in Flemming's or Hermann's fluid 

 to show spermatogenesis. 



