XXXV] TISSUES OF REPRODUCTION 285 



Sections of the testis on the cover-slip may be stained in 

 saffranin and then by Hermann's gentian violet method (cp. 

 p. 289) ; the sections are left in the gentian violet for 2 to 

 3 hours. 



Film preparations may be made of spermatozoa, and these 

 may be (a) dried or (6) fixed in formol and alcohol (cp. p. 28) or 

 in mercuric chloride or in 1 p.c. osmic acid. 



The films may be stained by the iron-haematoxylin method 

 (cp. p. 287) or with gentian violet (p. 289), or with acid magenta. 



