APPENDIX. 



I. OBSERVATION OF FRESH TISSUES. DISSOCIATION. 

 TEASING. 



A. Observation of fresh tissues. All tissues 

 should so far as possible be examined in the living 

 state. 



In the frog, and other small cold-blooded animals, 

 the gastric glands (cf. p. 169), pancreas, edge of the 

 liver, tongue, and mylo-hyoid muscle can be seen with 

 the circulation continuing. In the newt the kidney 

 and spleen can also be observed. In the rabbit the 

 pancreas, the middle lobe of the parotid gland, the thin 

 platysma muscles of the skiri can be seen with the 

 circulation. The tissues in the mammal should be 

 irrigated with 1 p.c. salt solution at about 39 C. 



The living appearance is preserved for a time after 

 the tissue is removed from the body. A small piece of 

 the tissue should be taken and examined as quickly as 

 possible, without the addition of fluid. 



Small pieces may be obtained, 



a. by cutting free-hand with a razor ; this method 

 may be used if the tissue is fairly firm ; 



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