302 APPENDIX 



When used for staining medullated fibres (p. 239, 7), 2 to 

 5 c.c. of formol is added instead of 10 c.c. 



Picric acid and nitric acid. 



Make a cold saturated solution of picric acid in water. To 

 100 c.c. of this add 2 c.c. of concentrated nitric acid. 



Picric acid and sulphuric acid (Kleinenberg). Sulphuric 

 acid is used instead of nitric acid in the preceding mixture. 



Picric acid and mercuric chloride. Equal parts of 

 saturated solutions of the two substances (for spinal ganglion 

 cells) ; other proportions are used in special cases. 



Alcohol and chloroform. (Oarnoy, v. G-ehuchten.) 



Absolute alcohol 60 c.c. 

 Chloroform 30 c.c. 



Glacial acetic acid 10 c.c. 



The mixture penetrates well. It has been modified as follows : 

 absolute alcohol 80 c.c. ; chloroform 15 c.c. ; acetic acid 5 c.c. ; 

 mercuric chloride 20 grams. The hardening is rapid. 



