APPENDIX 307 



IV. STAINING FLUIDS. 



Preservation of staining fluids from fungi. A fragment 

 of camphor or thymol should be added to all aqueous solutions 

 of stains. 



Ehrlich's acid haematoxylin. 



Haematoxylin 2 grms. 



Absolute alcohol 100 c.c. 

 Water 100 c.c. 



Glycerine 100 c.c. 



Glacial acetic acid 10 c.c. 



Alum to saturation. 

 Delafleld's haematoxylin. 

 4 c.c. of a saturated solution of haematoxylin in absolute 



alcohol. 

 150 c.c. of a solution of ammonia alum saturated in the 



cold. 

 Leave for a week, filter, and add 



25 c.c. methyl-alcohol. 

 25 c.c. glycerine. 

 Hsemalum (Mayer). Add 



1 gram haematein, to 

 50 c.c. of 90 p.c. alcohol, 



and dissolve by warming, pour into 

 1 litre of a 5 p.c. solution of alum. 

 Leave two or three days, and filter. 



Time of maximum staining power. Delafield's haematoxylin 

 becomes darker and increases in staining power for several 

 months after it has been prepared ; the acid hsernatoxylin 

 increases in staining power for a month or two ; the haemalum 

 is at its maximum or nearly so, when first prepared. 



Dilution of hcematowylin. It is best to dilute the several 

 fluids, when they stain too quickly, with a mixture of the 

 constituents of the fluid minus the haematoxylin, but when 



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