310 APPENDIX 



intestinal canal (cp. p. 326) ; and saffranin for staining dividing 

 nuclei (cp. p. 286). 



Nissl's methylene blue. 



Methylene blue 3 '75 grms. 



Venetian soap 1*75 



Water 1 litre. 



For method of using cp. Lesson xv. p. 117. 



Loffler's methylene blue. 30 c.c. of a saturated solution 

 of methylene blue in absolute alcohol, and 100 c.c. of '01 p.c. 

 caustic potash. 



Eosin. The solution is made as in p. 309, c; cp. p. 17 

 (oxyphil granules); pp. 40, 117 as diffuse plasma stain; p. 41 

 orange stain for red blood corpuscles ; p. 67, staining in bulk. 



Erythrosin (Held). 



Erythrosin 1'Ogrm. 



Distilled water 150'0 c.c. 

 Glacial acetic acid 2 drops. 



Ehrlich-Biondi stain. (Cp. p. 42.) It is best to get from 

 Griibler of Leipzig a mixture of solid methyl -green and orange G, 

 and make up as he directs. 



It is perhaps best used with tissues hardened in mercuric 

 chloride or alcohol, or for a short time in Flemm ing's fluid. 



Acid magenta and picric acid (van Gieson). 



95 p.c. alcohol 200 c.c. 



Picric acid 2 grms. 



Acid magenta (Rubin S) 0'2 grm. 



It is best used after rather deep staining with hrcmatoxylin 

 (cp. p. 41). Sections after staining with hsematoxylin are left 

 in this fluid for half a minute to 2 or 3 minutes ; if left too long 

 the acid magenta overstairis, and the hEematoxylin tint is apt to 

 be destroyed. The sections are washed with water (15 to 30 

 seconds), dehydrated, and cleared with cedar- wood or origanum 

 oil. To avoid too great a loss of colour, the water and the 

 alcohols may be made a faint red by adding a drop of the staining 

 mixture 



