APPENDIX 311 



Aniline blue and orange G (Mallory). 



Aniline blue soluble in water 0'5 gram. 

 Orange G 2'0 



Oxalic acid 2-0 



Water lOO'O c.c. 



The tissue is fixed in mercuric chloride, sections may be 

 imbedded and stained on the cover-slip, or cut frozen and 

 stained. Place in 



(a) 1 p.c. aqueous phosphomolybdic acid for 1 or more 

 minutes, and wash well in water. 



(6) Aniline blue mixture for 2 to 20 minutes. Dehydrate 

 in alcohol (best rather quickly), clear in xylol. 



Connective tissue fibres are stained deep blue, mucin and 

 amyloid substance a light blue ; blood corpuscles, keratin and to 

 a certain extent nuclei and muscle are stained yellow. The 

 staining is very sharp and very effective. 



Triple stain. The section may be stained for 1 to 3 minutes 

 in ! to '5 p.c. acid magenta, and washed in water before being 

 treated as above. 



Carmine, picric acid and indigo carmine (Calleja). 



(1) Carmine 2 grams. 

 Sat. aqueous lithium carbonate 100 c.c. 



(2) Indigo carmine 0*25 gr. 

 Sat. aqueous picric acid 100 c.c. 



Sections are placed in fluid (1) for 5 to 10 minutes, in acid 

 alcohol for 20 to 30 seconds (till pale red) ; and washed well 

 with water. They are then placed in fluid (2) for 5 to 10 

 minutes, in acetic acid '25 to '5 p.c. for a few seconds and washed 

 well in water. They are finally placed in alcohol to dehydrate 

 and take out excess of picric acid ; and cleared with xylol. 



The method gives good results with connective tissue and 

 epithelial cells. 



Orcein (Unna's method). 



100 c.c. absolute or 95 p.c. alcohol. 

 1 gram orcein. 

 1 c.c. hydrochloric acid 



