328 APPENDIX 



IX. INJECTION MASS. 



a. Place 20 grms. of gelatine in cold water until it is well 

 swollen, then pour off the water, and place the gelatine in a 

 water bath at about 40 C. (covering it up to prevent evaporation) 

 until it forms a fluid mass. 



Eub 8 grms. of carmine into a paste with water, add about 

 10 grms. of strong ammonia and mix well, then add about 100 c.c. 

 of water (or 2 p.c. chloral hydrate if the mass is to be kept), 

 shake well and filter ; if a suction-pump is not used it will 

 probably take 10 to 20 hours to filter. Warm the filtrate to 

 about 40 C. Pour it then slowly into the gelatine kept warm 

 over a water bath, stirring continuously; when the fluids are 

 well mixed, add gradually first strong acetic acid and then acetic 

 acid diluted 10 times, stirring as before, until the smell of 

 ammonia gives way to a faint smell of acetic acid. Strain 

 through new flannel washed with warm water. 



6. Prepare gelatine as in (a). Take 100 c.c. of a 2 p.c. solution 

 of Berlin blue warmed to 40 C. and pour it slowly into the gelatine 

 kept warm on a water bath, stirring continuously. 



c. 10 grams of gelatine are placed in 40 c.c. of water, allowed 

 to swell, then warmed to solution ; to this 10 c.c. of a solution of 

 bichromate of potash, saturated in the cold, are added. The 

 mixture is warmed to about 90 C. ; and to it is added slowly, 

 stirring the while, 10 c.c. of a neutral lead acetate saturated in 

 the cold and also at about 90 C. This should be made as 

 required. 



The gelatine mixtures must of course be injected warm ; the 

 blood should be washed out of the organ to be injected with 

 warm salt solution ; during the injection warm salt solution 

 should be poured over the organ or the whole animal should be 

 immersed in warm salt solution. 



