DR. LIONEL BEALES BIOPLASM STAIN. 267 



Carmine 10 grains. 



Liquor ammonias drachm, 



Glycerine 2 oz. 



Distilled water 2 oz. 



Alcohol ., .. | oz. 



The carmine is to be dissolved in the ammonia, boiled 

 for a few seconds, and set aside for an hourj the glycerine, 

 water, and alcohol may then be added, and the whole 

 allowed to stand until thoroughly settled, when the clear 

 fluid is to be decanted and kept for use. Dr. Beale tells us 

 that if the solution be very alkaline, the colouring will be 

 too intense, and much of the soft tissue round the bioplasm 

 will be destroyed by the action of the ammonia ; if, on the 

 other hand, the solution is neutral, the uniform staining of 

 tissue and bioplasm may result. In some cases the fluid 

 must be diluted with alcohol, water, or glycerine, in order 

 to get the best results; and Dr. Beale observes that the 

 process should not be hastily condemned without trying 

 the effect of modifying the quantities of the various 

 constituents. 



There are many other processes to be found scattered 

 through the pages of microscopical literature, but space 

 compels us to close the subject here. 



The process of injecting should now claim our attention, 

 though it is feared that actual practice with the syringe 

 will be found of far more use than any written instructions 

 can ever be. 



There are two methods of making injections, opaque and 

 transparent ; in the former the colouring matters, such as 

 vermilion, chromate of lead, white lead, or Prussian blue, 

 are well mixed with size made of gelatine, of such a 

 strength that it will form a firm jelly on cooling. To make 

 the size, soak one ounce of gelatine in a pint of water over- 

 night, and dissolve with heat in the morning. 



The colouring matters must be mixed up in a mortar 



