INJECTING. 269 



Liq. ammonise 5 drops. 



Carmine 5 grains. 



Glycerine i oz. 



Acetic acid 8 drops. 



Glycerine I oz. 



Alcohol 2 drachms. 



Water 6 drachms. 



Dissolve the carmine in a few drops of water, and the 

 liquor ammonise at a gentle heat, and when dissolved add 

 half an ounce of the glycerine. Shake well, and then add 

 gradually half an ounce of the glycerine in which the acetic 

 acid has been dissolved, well shaking the bottle between 

 each addition. If not decidedly acid to litmus paper, a 

 few more drops of acid must be mixed with the remainder 

 of the glycerine, and added as before. Lastly, add the 

 alcohol and water very gradually, well shaking until the 

 whole is well mixed. 



The author does not intend to enter further into the 

 details of this branch of microscopy ; with the foregoing 

 solutions the student will be enabled to learn the elements 

 of the art, and if afterwards he has a desire to proceed 

 further, he will find full instructions for many fluids in ' Das 

 Mikroskop,' by Dr. Frey, or in the admirable treatise of 

 Dr. Lionel Beale. 



The operation of Injecting. The various steps in the pro- 

 cess of injecting are very similar, whether the object be 

 treated for the opaque or transparent methods, though in 

 the former instance it must be placed in warm water until 

 the temperature has been sufficiently raised. 



The necessary apparatus will be found as follows : 



A syringe with pipes and stopcocks. 



Conical corks. 



Dissecting knives (Fig. 153). 



Dissecting scissors (Figs. 154-56). 



Forceps (Fig. 158). 



Bull-nose forceps. 



Wash bottle (Fig. 223). 



