SECTION OF HUMAN LIVER. 113 



HUMAN LIVEE. 



' PKACTICAL DEMONSTRATION. 



The sections from which the illustrations have been drawn were 

 made from material hardened in Miiller's fluid. The tissue was then 

 cut, the sections washed by six hours' maceration in water, after 

 which they were treated successively with alcohol Nos*. 3, 2, and 1, 

 stained with hasma. and eosin, and mounted in dammar. This treat- 

 ment aids greatly in the demonstration of the blood capillaries, as 

 the contained blood-corpuscles, in consequence of some change 

 effected by the' chromium salt, take the eosin deeply. The nucleoli 

 of cells are also rendered markedly prominent. 



Pieces of tissue, a quarter of an inch square by half an inch thick, 

 may be hardened in alcohol. This method will give very excellent 

 results, providing the sections be cut as soon as the hardening process 

 has become complete. Stain as above. 



For the demonstration of the isolated hepatic cells, scrape the cut 

 surface of a piece of hardened liver with a scalpel, and throw the 

 scrapings into a watch-glass of haema. After a few moments, drain off the 

 stain, and brush the stained tissue elements into a test-tube nearly 

 filled with water. Change the water two or three times; and when 

 clear, add a few drops of eosin solution. Allow the eosin to stain for 

 a moment only; decant, drain, and fill the tube with alcohol. After 

 ten minutes, the spirit may be drained off, and the tube partly filled 

 with oil of cloves. A drop of the sediment may then be placed upon 

 the slide, the bulk of the oil removed with paper, and the mounting 

 completed by adding a drop of dammar and the cover glass. I am in 

 the habit of keeping this tissue in the oil, from year to year, for use 

 in my classes. If the oil be pure, and the washing thorough, the 

 staining will remain unaffected for certainly two or three years. 



SECTION OF HUMAN LIVER. 



Cut at right angles to the surface, and stained with hsema. and eosin. 



(Fig. 80.) 

 OBSERVE : 

 (L.) 



1. The imperfectly outlined lobules (in consequence of the ab- 

 sence of interlobular connective tissue). 



2. The fusing of the lobules. (At points like B B, it is impos- 

 sible to say just where one lobule ceases and the contiguous one 

 begins.) 



3. The central (or intralobular) veins A A (frequently appearing 

 as mere slits on account of the direction of the cut). 



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