APPENDIX 473- 



(2) Apply the guaiac and ozonic ether test. 



When it is desired to ascertain whether Ferments be present it 

 is necessary to add a piece of coagulated egg white, or of washed fibrin 

 to the original fluid, and to place the mixture on a water bath heated 

 to body temperature. If, after an hour, the digest gives a distinct 

 proteose reaction, and this was not obtained in the original fluid, the 

 presence of a proteolytic ferment may be assumed; pepsin, if the 

 original fluid react acid, and trypsin, if it react alkaline. If proteoses 

 are present in the fluid itself, Mett's method must be employed to 

 identify the ferment. 



For the detection of Amylolytic and Steatolytic ferments, the 

 methods described in the text must be employed. 



For the detection of the various substances which may occur in the 

 urine, the test and reactions described in the text must be applied. 



