CUTTING l W' MOUNTING 8ECTI0N3. 



a water-bath, in order to evaporate tin oil. The 



secti< : - may next It freed from the embedding and 



colored according t<> desire. Gag< recommends thai 

 the collodion ami clove oil be applied separately.* 

 "The paraffine is now removed ami the sections are 

 stained, generallj with borax-carmine, \\ 1 1 i « * 1 1 two operations 

 an- performed as follow s: A series of glass i ubes large enough 

 to hold a Blide is filled with the following reagents, and 

 arranged in tin- following order: Turpentine (or naphtha); 

 absolute alcohol; f" 1 percent alcohol; 70 per cent alcohol; 

 alcoholic borax-carmine; i<> per cent alcohol acidulated with 

 IK 1: '.mi per cent alcohol: absolute alcohol. The slide. 

 having been warmed to the melting-point of the paraffine, 

 is plunged into t he turpent ine, which removes the paraffine; 

 then passed through the tubes with the successive alco- 

 hols into tip' stain, from which it is brought into the suc- 

 ulcohols <>f the ascending . which wash out the 



stain and dehydrate the Nothing mo i re- 



mains to be done hut to treat the sections with a drop of ben- 

 zol or turpentine, ami to add Canada balsam ami . ing- 



glass/ 



When the objects are small and sufficiently permea 

 t he sections can he stained on the slide. •• In this case the 



object after having been fixed and washed out is taken 

 wl ile .-till on its way through the lower alcohols (it should 

 no- be allowed to proceed to the higher of alcohol 



before Btaiuing) and passed through a hath of alcoholic 

 borax-carmine (or other alcoholic stain) of suffiei< nt dura- 

 tion, then dehydrated with successive alcohols, pas 

 through chloroform into paraffine, and cut a- al 



described. " \ 



* See Whitman's Methods of Research in Mi J Anatomy 

 and Embryology, pp. 121, 122. 



+ lf the objects have already been soaked ii oil, or other 



Qtial oil, for tlie purpose of clearing, they ma nabedded 



direct from the clove-oil, or this may be n by means 

 chloroform, which is the better practice I., 



