

: TING AND MOU& l /.\ 



in strong alcohol and allow it - lain until it 

 cut, changing hoi if i y. 



When read] 

 the well of the microtome, with tl s in the 



well, and (ill the well even to the surface with the paraffine 

 embedding mi n as the paraflfine bai 



slide can be easilj removed with a sliding motion which 

 leaves the surface to be cut parallel to the surfaci 

 microtome. 



Cut away the embedding mass of paraflfine in such a man- 

 ner that the front and back sides shall be parallel and leave 

 enough t<> support the object to be cut, and then slice with 

 a razor or the knife of the microtome. The parallel i 

 of the first Bection adhi of the ling 



Bection, and the series of sections can be d from the 



knife placed in order on thi ed in position. 



The bu] an be removed with warm tur- 



pentine, and the turpentine with alcohol if it rable 



-;ain i he seel ions on the slide. 



I E t li«' piece to I ioned is 1 i ith 



the solution of celloidin it can be embedded in a small 

 folded paper box, as directed for embedding in pan 



A- both absolute alcohol and oil of <■!■ the 



celloidin, in dehydrating use alcohol of 96 pi . and in 



clearing oil of bergamot, oil of sandal, or oil <>f origanum.* 



Dr. I.. Low* - mixture for embedding is the followiu 



1 latine l p 



Glycerine 1 ; 



W ater 5 



This i- put upon tlu- water-bath and thoroughly 

 and afterwards strained. After coloring tl 

 aration it is well washed with water ami tl 

 in the gelatine at a temperature of not n 122 I'. 



* Contributed 1>\ N. \. Mas 



