i YD MOUNTING 319 



results. For fixing the tissues the author used the mix! 

 mended bj Gilson and Carnoy, and with the ult, and 



Flemming's fluid. (Archiv fm Mikr. An:; also Journ. 



Roj Mil r. 8o< , I 590, 1078 



Making Sections of Eggs- Th mould, accordii 



skj - method, be placed, w ben alive, for a short time in nearly boiling 

 water, and then hardened in bichromate of potash. They may be 

 Btained in picrocarmine or in hematoxylin, and embedded for cut 

 ting in paraffinc or coagulated albumen. Patten placed thi 

 Trichoptera in cold water, raising the temperature very gi iduaJly i<» 

 about s <> < . or when they became hard and while; then remo\ 

 20 per cent alcohol, which \\a< increased l»\ 10 per cent once or twice 

 a day until reaching full strength (96 per cent). They were stained 

 with Klcinenberg's hematoxylin and ;i 7<> percenl solution of cochi- 

 Deal, and embedded in paraffine after being clarified in benzol 

 thirty minim - 



\\i:l;u/il examined the embryos of the viviparous Aphides in a weak 

 Ball solution 1 per ci nt), in which thej live for about an hour, 

 earij in the development of 1 in- eggs may tx 



treatment \\ ith hydrochloric acid i "> pi r cent p i<- acid, as thi se 



nts partial!) dissolve the yolk elements, rendcrin paration 



transparent; but the lafc paque by 



ihis treatment 



I radually hardened in alcohol and then cut ai the 



slide with picrocarmine or hematoxylin oj lions. 



Cowalevsky's sections, made before the days of mien 

 cut i>y band, the eggs ha\ ing been, after being bardi m d, embedded in 

 paraffine and cut with a razor. 



Dr. F. Stuhlmnn* in the examination of the eggs of insi 

 spiders, Myriopods, and Peripatus, examined fresh objects in 

 percent sail solution, to which is sometimes added weak acetic and 

 methyl-green acetic acid. The foregoing was ible for 



youj g eggs, as oldi r ones are too opaque 

 centrated sublimate solution proved the best. \\ 

 alcohol, and hot sublimate solution were n< 

 sublimate fixed in ."i 1,, in minutes. The preparations 

 oughly washed ; a f.u drops of tincture of iodine hi 

 cess. Then 60 per cent spirit, and finally absol 

 chorion is perforated with a fine needle, but the 

 avoided. Ovaries arc placed forseveral hours iu 1 rm, 



from one to three days (according to size) in 



•Ber. Naturf. Gesell. Freiburg, i. i: 1 L881 R Micr 



Boc , April. 1887. 



