VITALITY OF PUTKEFACTIVE OKGANISMS. 143 



Fig. 14. 



temperature of 90° Fahr. On the morning of the 8th 

 of October (that is to say, two days after their pre- 

 paration) the infusion in every one of the bulbs was 

 turbid and covered with scum. 



Here once more we have 'perfect harmony bettveen 

 my results and those of Dr. Roberts and Professor 

 Gohn. 



On the 2nd of October, moreover, fourteen of our 

 ordinary small retort-flasks with bent necks (shown 

 in fig. 14) were charged with 

 the neutralized hay-infusion. 

 They were boiled for three mi- 

 nutes, and hermetically sealed 

 whilst boiling. Some days after- 

 wards one tube of the entire 

 number was observed to have be- 

 come lighter in colour and sen- 

 sibly cloudy ; but thirteen out of 

 the fourteen remained unchanged 

 in colour, brightly transparent, 

 and entirely free from life. 



Here the dissidence between my results and those 

 of Professor Gohn, who also experimented with her- 

 metically-sealed flasks, reappears. 



Numerous other experiments with pipette-bulbs and 

 retort-flasks were made at the time here referred to, 

 but it is unnecessary to record them. Suffice it to say 

 that, like those just described, some of them corrobo- 

 rated and some of them contradicted the results of Dr. 

 Eoberts and Professor Cohn. 



