VITALITY OF PUTEEFACTIVE OEaANISMS. 191 



here aimed at was that the liquid should be discharged 

 into the bulb far below the india-rubber connecting- 

 piece, and that during tlie discharge it should pass only- 

 through filtered air. 



Fig. 19. 



Each bulb was exhausted in the manner already 

 described, and refilled three times in succession. When 

 last filled it was plunged for a minute or so into 



