VITALITY OF PUTEEFACTIVE OKGANISMS. 219 



ally sealed and boiled as before, six out of the seven 

 remain as clear as distilled water. 



On the 20th of March seven bulbs were charged with 

 infusion of cucumber, and subjected to the action of the 

 Sprengel pump for seven hours. They were afterwards 

 treated in the manner just pj^ 21. 



described. They were all ^^^^ gs r ^^^-- -,,^1- '^;^ w 

 completely sterilized. 



On the 27th of March 

 three bulbs were charged 

 with cucumber - infusion 

 and subjected to the ac- 

 tion of the Sprengel pump 

 for five hours. One of them 

 was subsequently boiled for 

 five minutes, another for 

 one minute, while the third 

 was left unboiled. This 

 third tube became faintly 

 cloudy, but the two others 

 remain perfectly free from 

 life. 



This result invited re- 

 petition. On the 29th 

 accordingly six bulbs of 

 cucumber - infusion were 

 exhausted for five hours, 

 and afterwards sealed and 

 boiled for a single minute. 

 Five of the six bulbs re- ^'^^^^^ 

 mained permanently clear ; one became cloudy. 



On the 30th of March six bulbs containing turnip- 

 infusion were exhausted for five hours and boiled after- 

 wards for a minute. Five remain perfectly clear, one 

 has become muddy. 



