160 



ESSENTIALS OF CHEMICAL PHYSIOLOGY 



The following table represents these facts concisely : 



7. Pigments of Muscle : — 



(a) Notice the difference between the red and pale muscles of the rabbit. 



(6) Examine a piece of red muscle (e.g. the diaphragm) spectroscopicaUy 

 for oxyhsemoglobin (or it may be more convenient to make an aqueous 

 extract of the muscle and examine that). 



Via. 61. — 1, Absorption spectrum of myohffimatin, as seen in muscle rendered transparent by 

 glycerin. 2, Absorption spectrum of modified myohasmatin . 



(c) A piece of the pectoral muscle of a pigeon has been soaked in glycerin. 

 Press a small piece between two glass sides and place it in front of the spec- 

 troscope. Observe and map out the bands of myohsematin. 



(d) Pieces of tiie same muscles have been placed in ether for twenty-four 

 hours. The ether dissolves out a yellow lipochrome from the adherent fat. 

 A watery fluid below contains modified myohsematin. Filter it ; compare 

 its spectrum with that of hismochromogen. The myohsematin bands are 

 rather nearer the violet end of the spectrum (fig. 61, spectrum 2) than those 

 of hsemochromogen (fig. 57, spectrum 9). 



8. Creatine : — 



(a) Take some of the red fluid described in 7, d, and let it evaporate to 

 dryness in a desiccator over sulphuric acid (fig. 62). 



' This proteid appears to be absent in many forms of involuntary muscle- 



