404 EXCRETION 



Heat in the water bath for 20 minutes. Upon cooling a deposit of yellow 

 crystals of phenyl-glucosazone takes place, if glucose is present. 



1 8. Quantitative Determination of Sugar in the Urine. Fill a 10- 

 c.c. graduated pipet with freshly prepared Fehling's solution. Take 10 c.c. 

 of urine, measured with a dropping-pipet into a small beaker, and boil. While 

 continuing to boil, add Fehling's solution slowly and cautiously so long as 

 the color is discharged. The amount of Fehling's required to reduce the sugar 

 is a measure of the quantity of reducing sugar present i c.c. of Fehling's 

 being equivalent to 5 milligrams of dextrose. 



For the presence of blood pigments and other abnormal constituents of 

 the urine, the student is referred to special handbooks on the subject. 



