396 



BLOOD AND LYMPH. 



of oxyhemoglobin (less than 0.01 per cent.) the /5-band is lost and 

 the a-band is very faint in layers one centimeter thick. With 

 stronger solutions the bands become darker and wider and finally 

 fuse, while some of the extreme red end and a great deal of the violet 

 end of the spectrum is also absorbed. The variations in the absorp- 

 tion spectrum, with differences in concentration, are clearly shown 

 in the accompanying illustration from Rollett * (Fig. 171) ; the thick- 



660 



c r> e 



650 6k0 630 620 610 600 sk) 580 570 560 550 5k0 53d\ 520 



I. 1....I....1....I ll. l tl ll ,Jill l mlltilll.Mlltnl|.|JtllllHMllllllLllllllllllllL l .llllllll.llllJl 



mo 



J 



Fig. 170. Table of absorption spectra (Ziemke and Mutter) : 1, Absorption spectrum 

 of oxyhemoglobin, dilute solution; 2, absorption spectrum of reduced hemoglobin; 3, ab- 

 sorption spectrum of methemoglobin, neutral solution ; 4, absorption spectrum of met- 

 hemoglobin, alkaline solution ; 5, absorption spectrum of hematin, acid solution ; 6, ab- 

 sorption spectrum of hematin, alkaline solution. 



ness of the layer of liquid is supposed to be one centimeter. The 

 numbers on the right indicate the percentage strength of the oxyhem- 

 oglobin solutions. It will be noticed that the absorption which 

 takes place as the concentration of the solution increases affects 

 the red-orange end of the spectrum last of all. 



Solutions of reduced hemoglobin examined with the spectroscope 

 * Hermann's "Handbuch der Physiologie," vol. iv, 1880. 



