PREFACE TO THE SEVENTH EDITION 



After a lapse of three years the entire volume has been thoroughly 

 revised, in an attempt to bring it strictly up to date. To obviate the 

 necessity of further enlarging the book, many methods and tests 

 which the author believes to have outlived their usefulness have been 

 omitted, in order to afford space for more modern procedures. Inas- 

 much as "blood counting" is not a part of the greater number of courses 

 in physiological chemistry, all discussion of this subject has been elimi- 

 nated. Among other changes in the carefully revised chapter on 

 ''Blood Analysis" the excellent analytical system of Folin and Wu has 

 been included in its entirety. No course is complete without a con- 

 sideration of these thoroughly up-to-date methods. The chapter on 

 "Acidosis" has been expanded to include a brief consideration of 

 respiration and is now called " Respiration and Acidosis." The chapter 

 on "Quantitative Analysis of Urine" is introduced by a discussion of 

 Normal and Standard Solutions and their Method of Preparation. 

 Procedures similar to those given here in concise form are used in the 

 best medical schools in the country as an introduction to their practical 

 courses in physiological chemistry. In line with the increasing know- 

 ledge and importance of Vitamines, considerable space has been given 

 to this subject in the section on Metabolism. . In this connection sug- 

 gestions are made as to suitable ways of demonstrating various "diet- 

 ary deficiencies." The proper way to cage and care for animals used 

 in such experiments is also discussed. 



As usual, the author is under great obligations to his confrere, Dr. 

 Olaf Bergeim, for invaluable assistance in all matters pertaining to 

 revision and proof. Dr. Clarence A. Smith has also rendered valuable 

 aid in connection with this new edition. The author also wishes to 

 thank Miss Katherine Loewinson, Dr. Raymond J. Miller, and Mr. 

 Bernard L. Oser for assistance. 



It is a pleasure to acknowledge the many courtesies extended to the 

 author by the publishers of this volume. 



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