596 



PHYSIOLOGICAL CHEMISTRY 



5. Influence of Energy Deficiency. The bulk of the energy in the 

 accustomed diet of man is furnished by carbohydrates and fats. There- 

 fore, if we reduce the dietary content of these substances to the mini- 

 mum and maintain other dietary factors (vitamines, protein, salts, 

 water, etc.) at a normal level, we will not be properly nourished. The 

 body will attempt to derive the necessary energy by the combustion 

 of body tissues and a pronounced and rapid loss in body weight accom- 

 panied by other signs of abnormality will soon follow. 



Demonstration on Energy Deficiency. (a) Demonstration on man, following 

 the suggestions embraced in the preceding paragraph. 



(b) Demonstration on the White Rat. Place two young white rats (100-150 

 grams) in individual cages. Feed one the adequate diet listed as Diet i below. 

 Feed the second animal the same diet minus the carbohydrate. This animal 

 will lose weight rapidly, due to the low energy value of the diet whereas the other 

 rat will grow normally. 



ENERGY DEFICIENCY DIET 



6. Influence of Inorganic Matter (Calcium) Deficiency. A demon- 

 stration of the harmful effect following the elimination of calcium from 

 the diet may readily be made if the diets listed below be fed to young 

 white rats. 



DEFICIENCY OF CALCIUM DIET 



liver contains Water-Soluble B and Fat-Soluble A The diet is adequate except 

 for calcium (McCollum, Simmons, Parsons, Shipley, and Park; Jour. Biol. Chem., 45, 

 333, 



