PREPARATION OF BACTERIAL ANTIGENS 





served the purpose of establishing the principle of complement 

 fixation in a specific antibody-antigen reaction, they were 

 soon found by Moreschi 4 to be unsatisfactory because the 

 bacterial cells so suspended often of themselves so markedly 



T /7 ff L. T TT 



COMPAfllSON OF AGGLUTINATION VALUE* OF SERA FROM ffABB ITS INOCULATED WITH 

 CftJ ORC* Ni SMS FROM fftOTEIN FffEE MEDIUM, AND 

 CBJ SAME QRSflMISMS FROM PROTEIN COHTfl/N/NC MEDIUM 



inhibited the action of the complement as to disallow a con- 

 clusion concerning a true fixation of the complement in a 

 specific antibody-antigen reaction. 



That the complication could be minimized by the substitu- 

 tion of extracts of the bacteria for a suspension of the cells 

 was demonstrated in a considerable series of experiments by 



