KESULTS OF EXAMINATIONS. 21 



the crystals softened at 144, melted at 151 to 152, and were com- 

 pletely liquid at 1 58. Analysis of the silver salt of the crystals gave : 



Per cent. 



0.4689 grain silver salt gave 0. J'22(J grain of Ag or 26. 15 



Calculated for Ag (C^^O^ 26. 37 



The crystals from the crude oleoresin were next examined. A 

 portion of the white crystalline mass which had settled to the bottom 

 of the container was pressed in a hand press, and nearly all the liquid 

 constituents were thus removed. Another method was found to give 

 equally satisfactory results: The crude resinous mass was thrown 

 into a wide Biichner funnel, using silk bolting cloth as a filter; the resin 

 was spread out in a thin layer and suction applied. After two hours 

 a white crystalline mass remained, no longer sticky to the touch, and 

 practically free from volatile oil. After several crystallizations from 

 acetone, finally from methyl alcohol, the crystals melted at 131; 

 a higher melting point was not obtained. The alcoholic solution 

 of the crystals was strongly Z-rotatory. The specific rotation, 

 [a] D = 95.82, was determined from the following values: 

 a= -3.74 p = 4.764 



1= 1 d= .8193 



Some of the crystals were dissolved in alcohol and a few drops of 

 concentrated hydrochloric acid added. On cooling, crystals were 

 obtained, in the form of triangular plates, which melted at 158 to 

 159. (See PL I.) The silver salt of these crystals gave, on analysis, 

 the following results: 



Per cent. 



0.2840 gram silver salt gave 0.0751 gram silver =26. 44 



Calculated for Ag (0^2962) =26.37 



CONCLUSIONS. 



The volatile oil from Pinus sabiniana consists almost entirely of 

 normal heptane. The rosin probably consists of a noncrystallizable 

 form of abietic acid. The rosin distilled under reduced pressure and 

 the crude oleoresin furnished crystalline resin acids of the formula 

 C 2 oH 30 O 2 , and it may be assumed that the rosin consists of amorphous 

 acids of the same composition. 



BIBLIOGRAPHY. 



(1) BLASDALE, W. C. "On heptane from coniferous trees." Jour. Am. Chem. Soc., 



pp. 162-164, vol. 23, 1901. 



(2) KREMERS, E. "Oil from Pinus sabiniana." Pharm. Rev., pp. 165-172, vol. 



18, 1900. 



(3) RABAK, F. "Oleoresin of Pinus sabiniana." Pharm. Rev., pp. 212-215, vol. 



25, 1907. 



(4) SADTLER, S. B. "Abietene, a new hydrocarbon as type of a new group of 



terpenes." Am. Jour. Pharm., pp. 176-180, vol. 51, 1879. 



(5) SADTLER, S. B. "On heptane." Am. Jour. Pharm., pp. 293-294, vol. 51, 1879. 



