APPENDIX 



Rebipelagar or Neutral Red Bile Salt Agar: 



Agar 20 grams 



Peptone 20 grams 



Bile salt commercial 5 grams 



Water ICOO c.cms. 



Heat the ingredients in a double pan or autoclave until 

 completely dissolved; titrate with alkali and adjust the reac- 

 tion to +1.0 per cent to phenolphthalein. Cool to 45° C, 

 coagulate with egg albumen (5 grams dissolved in water), 

 heat to boiling, adjust the weight and filter. Tube in con- 

 venient quantities, after adding 5 grams of lactose and 5 c.cms. 

 of a 1.0 per cent solution of neutral red. 



Aesculin Bile Salt. (Harrison and Vanderleck, Trans. 

 Roy. Soc. of Canada, 1909, Sec. IV, 147.) 



Dissolve in water 1.0 per cent of Witte's peptone, 0.25 per 

 cent of bile salt, and 1.5 to 2.0 per cent of agar. Neutrahse 

 with alkali, coagulate with egg albumen and filter. Add 0.2 

 per cent of citrate of iron and 0.1 per cent of aesculin. This 

 amount of citrate of iron should give a final acidity of +0.7 per 

 cent and produces a slight fluorescence in the medium. 



Toissons's Solution: 



Methyl violet 0. 025 gram 



Sodium chloride 1.0 gram 



Sodium sulphate 8.0 grams 



Glycerine 30 c.cms. 



Distilled water 160 c.cms. 



The solution should be freshly filtered. 



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