URINE 307 



few cubic centimeters of distillate. An acetone urine will be 

 supplied containing sufficient acetone to give the tests. 



1. Rothera's Test. Saturate about 10 c.c. of urine with am- 

 monium sulphate. Add 2-3 drops of fresh sodium nitroprusside 

 solution and 2-3 c.c. concentrated ammonia. Mix and allow 

 to stand at least % hour. A characteristic permanganate 

 color indicates the presence of acetone. 



2. lodoform Test. Perform the iodoform test with acetone 

 urine. (See under Fermentation.) 



