TECHNIQUE. 57 



contrast stain, after which the sections are dehydrated 

 in 95 per cent, alcohol, cleared in oleum origani cretici, 

 and mounted in Canada balsam. As good results are 

 obtained by adding a few drops of the haematoxylin 

 solution to a small dish full of water containing the 

 sections and allowing them to stay in this solution 

 over night, after which the contrast stain is used as 

 described. 



Heidenhain's Iron-hcematoxyUn Method. Sec- 

 tions fixed with Zenker's fluid and not over 6 microns 

 in thickness may be stained by the Heidenhain method 

 in the same manner as heretofore described for stain- 

 ing smears of material containing amoeba?. 



A great deal of care is required in staining sec- 

 tions containing amoebae if the best results are to be 

 obtained, and sometimes many preparations have to 

 be stained before typical ones are secured. This is 

 true of any method which may be adopted, although 

 the writer believes that the Mallory method is the 

 easiest of application and gives the most uniformly 

 satisfactory results. 



For the diagnosis of amoebae in the stools stain- 

 ing methods should never be resorted to, as the 

 examination of fresh specimens is altogether superior 

 and safer. The stained preparations are only used 

 in the study of the morphological character and the 

 methods of reproduction of these organisms. 



