64 PARASITIC AMOEBAE OF MAN. 



TECHNIQUE OF CULTIVATION. A large number 

 of culture media have been recommended for the cul- 

 tivation of amoebse. Kartulis recommended a solution 

 of ordinary bouillon and sterilized infusions of hay; 

 Ogata used a 2^2 per cent, solution of grape sugar 

 in sterilized water; Vivaldi used sterilized straw in- 

 fusion, and Miller was able to grow the organisms 

 in a 1/5 per cent, solution of milk, in water, in hay 

 infusions, and in dilute bouillon. A number of ob- 

 servers have used solid media, such as agar, alkaline 

 potatoes, egg albumin, and 5 per cent, of Fucus cris- 

 pus in alkaline albumin. Among other media which 

 have been used may be mentioned Gensen's, com- 

 posed of barley sprouts cooked in water, filtered, 

 rendered alkaline, and mixed with sugar, after which 

 it was sterilized by the fractional method; Zaubitzer's, 

 consisting of solutions containing varying amounts of 

 somatose or agar containing from 1 to 2 per cent, 

 somatose; and Casagrandi and Barbagallo's, consist- 

 ing of the sterile white of egg containing bicarbonate 

 of soda. 



While it is true that free-living amoebge may be 

 cultivated upon almost all of these media the one 

 which has given the most satisfactory results is that 

 devised by Musgrave and Clegg, the formula of which 

 is as follows : 



