98 PARASITIC AMCEEM OF MAN. 



stained spherical or oval areas within the endoplasm. 

 With the Wright stain the nuclear chromatin 

 takes a bright red or crimson color and the nuclear 

 membrane stains very deeply, the chromatin situated 

 upon its inner surface appearing as bright red or 

 crimson masses. When over-stained the entire nucleus 

 is bright red and no morphological details can be 

 recognized. Within the substance of the nucleus 

 the chromatin may be observed as delicate threads, 

 rods, or granules, stained a bright red in color and 

 separated by unstained spaces. The karyosome 

 stains a deep red or violet and is usually well differen- 

 tiated. Just prior to division, during the vegetative 

 stage, the nuclear chromatin may be observed as two 

 crimson stained masses lying at the poles of the 

 elongated nucleus, but with this stain I have never 

 observed distinct evidences of mitosis. In specimens 

 stained with the iron-hasmatoxylin method evidence 

 of a primitive mitosis are sometimes observed. The 

 extrusion of the chromatin from the nucleus during 

 certain stages of reproduction, first described by 

 Schaudinn, is often evidenced in stained preparation 

 by the presence of minute deeply stained granules 

 of chromatin lying free in the endoplasm. The ap- 

 pearance of the nucleus in stained preparations dur- 

 ing the various reproductive stages will be found 

 described in the section dealing with this subject. 



