102 PARASITIC AMCEB^E OF MAN. 



In reproduction by schizogony complicated nu- 

 clear changes occur resulting in the formation of eight 

 daughter cells. The beginning of the process is first 

 evidenced by swelling of the nucleus apparently due 

 to the absorption of fluids from the endoplasm, while 

 at the same time the latter extrudes all foreign ma- 

 terial, and the entire organism becomes motionless. 

 The chromatin situated upon the inner surface of the 

 nuclear membrane divides into eight little heaps which 

 finally become free within the nucleus, and this is 

 followed by the rupture of the nuclear membrane 

 and the liberation of the eight daughter nuclei within 

 the endoplasm. After this occurs the cytoplasm of 

 the amoeba divides into eight more or less irregular 

 portions each containing one of the daughter nuclei 

 and in this way eight young amoebae are produced. 

 This process was first studied by Casagrandi and 

 Barbagallo and the plate reproduced from their work 

 well illustrates the daughter nuclei immediately after 

 liberation from the nucleus. 



In the living specimen the daughter nuclei are 

 visible as brightly ref ractile masses of granules due to 

 the chromatin of which they are largely composed, 

 while in stained specimens the chromatin takes a 

 bright red color when Wright's method is employed, 

 so that this method of reproduction can be easily 

 traced in such preparations. 



