AMCEBJE OF THE INTESTINAL TRACT. 133 



opposite being true of Entamceba coll. In the smaller 

 amoebae this distinction cannot be easily made, but 

 every specimen of feces containing this species will 

 show some organisms in which the distinctive staining 

 of the ectoplasm and endoplasm will be found. 



The nucleus of this species stains very poorly as 

 compared with that of Entamceba coli, on account of 

 the small amount of chromatin which it contains, as 

 well as from the fact that in many of the amoebae the 

 nucleus is undergoing division prior to sporulation. 

 With Wright's stain the nuclear chromatin stains a 

 pale red or pink, while if iron hsematoxylin be used 

 for staining the nucleus is dark blue or almost black 

 in color. As a rule, the nucleus appears larger in 

 Wright- or Giemsa-stained preparations and the 

 chromatin granules appear more irregular and 

 massive. 



METHODS OF REPRODUCTION. We owe our first 

 accurate description of the methods of reproduction 

 of Entamceba histolytica to Schaudinn, and upon the 

 difference in these methods from those of Entamceba 

 coll he very largely based his classification of these 

 two species. The difference in the methods of repro- 

 duction of Entamceba histolytica and Entamceba coli, 

 amply suffices to establish the two species, even though 

 we had no other evidence of their specific nature. 



Entamceba histolytica reproduces by simple divi- 

 sion by budding or gemmation, and by sporulation. 



