AMCEB.E OF THE INTESTINAL TRACT. 135 



but with the Wright or Giemsa stain it appears in 

 irregular masses. 



Reproduction by sporulation only occurs after 

 vegetative reproduction has taken place for many 

 generations. Until the researches of Schaudinn this 

 method of reproduction had not been observed in any 

 amoebae, but since his researches were published it has 

 been confirmed by numerous investigators and serves 

 to distinguish this species from the other intestinal 

 amoebse. In view of the interest attaching to Schau- 

 dinn's work I shall quote in full his description of 

 this method of reproduction. 



" As in many other parasitic protozoa the forma- 

 tion of these stages in Entamoeba histolytica occurs 

 only after a lengthy period of lively increase, when 

 the conditions of life have deteriorated. In dysentery 

 this is simultaneous with the commencement of heal- 

 ing. Permanent forms ensue when the feces become 

 firmer or in more correct language, healing begins 

 when the vegetative increase of the amoebae ceases. 

 During the height of the disease I have never found 

 these permanent stages. 



" The beginning of spore formation is first noticed 

 in the nuclear apparatus. The peripheral chromatin 

 zone of the nucleus broadens and extends into the 

 nuclear plasm, the nucleus at the same time becoming 



