AMCEBJS OF THE INTESTINAL TRACT. 137 



following results. The nucleus gives off chromidia 

 to the cytoplasm, which appear to multiply and scat- 

 ter through the entire organism, the nucleus degen- 

 erating and either entirely dissolving or being ex- 

 truded. The chromidia withdraw from the endoplasm 

 and collect in the dense fibrous tissue of the ecto- 

 plasm, finally permeating the latter as a uniform 

 reticular chromidial mass. Ectoplasma buds filled 

 with a chromidial mass then protrude upon the sur- 

 face of the parasite, and are finally budded off from 

 the parent body. As soon as the sheath of these 

 globules is formed staining substances no longer act 

 well. 



"If the yellowish brown sheath now forms, even 

 the stained preparations will no longer give informa- 

 tion concerning the structure of the interior of the 

 globules, and section technique likewise fails, for the 

 small globules do not yield to the knife. Hence I 

 am unable to state anything in regard to the nuclear 

 changes occurring in the interior of these spores." 



Prior to the observations of Schaudinn regarding 

 this method of reproduction, I published a description 

 of Entamceba histolytica in which I mentioned the 

 occurrence of bodies within the cytoplasm which I 

 thought might be spores. In concluding this paper I 

 said: 



