138 PARASITIC AMCEB.E OF MAN. 



" There occur in all but the degenerative forms 

 of the amoebae small round or oval, dimly stained 

 areas, uniform in appearance, and most numerous in 

 the large full-grown forms, being entirely absent in 

 the vacuolated shells of amoeba?. These areas re- 

 semble similar areas in stained segmenting malarial 

 plasmodia, and which are in them due to the young 

 spores. Reasoning from analogy it may be that these 

 areas in the amoebae are also spores." 



After the appearance of Schaudinn's work it was 

 very apparent that the bodies which I described were 

 in reality the small masses of chromatin which form 

 a portion of the nucleus of the young spores. 



I have been able to confirm Schaudinn's descrip- 

 tion of this method of reproduction and the following 

 description gives the results of my studies of this 

 process as observed in living and stained preparations 

 of Entamceba histolytica, using both wet and dry 

 fixed specimens. 



It is not unusual to observe in the f eces amoebae in 

 which the endoplasm contains refractile granules and 

 rods collected in irregular masses or distributed 

 throughout the organism. These bodies are the 

 chromidia which have been liberated by the breaking 

 up of the nucleus. Werner considers that part of the 

 chromidia is derived from the peripheral nuclear 

 chromatin and part of it is produced by the karyo- 



