164 PARASITIC AMOEBAE OF MAN. 



preparations under cover glasses. These preparations 

 were carefully examined, the examination being con- 

 ducted upon a mechanical stage and requiring many 

 hours. No forms resembling the cysts of Entamceba 

 coli were found, but the small spores of Entamceba 

 Mstolytica were noticed in large numbers, but no 

 vegetative organisms could be demonstrated. The 

 cover glasses were then removed, the feces washed 

 with distilled water, and ten such preparations were 

 mixed with enough distilled water to form 1 c.c. of 

 the mixture. The feces of the animal to be ex- 

 perimented upon, a healthy, strong young cat, was 

 carefully examined for amoebae and none could be 

 demonstrated. To this cat I gave the 1 c.c. prepara- 

 tion mentioned above, mixing it with milk. On the 

 evening of the third day the cat passed bloody mucoid 

 feces and an examination showed the presence of 

 great numbers of typical Entamoeba Mstolytica. In 

 the afternoon of the fourth day the cat perished. 

 Dissection showed typical ulcerous dysentery of the 

 large intestine, and immigration of the amoebse into 

 the epithelium could be easily established. 



" I will mention yet another experiment which 

 goes to prove that the permanent spores by them- 

 selves are capable of producing a new infection. 

 The feces of the cats developing dysentery con- 

 tained only vegetable stages of the amoebae, no spores 



