176 PARASITIC AMCEBJE OF MAN. 



testinal coats. The amoebae observed in the feces and 

 in the sections of intestine presented the morpho- 

 logical characteristics of Entamceba Mstolytica in 

 every case. 



Control Experiments. All the kittens experi- 

 mented upon were very carefully examined for 

 several days prior to infecting them for the presence 

 of amoebae, and all were found negative, so that there 

 can be no question of a previous amoebic infection. 



That amoebae produced the lesions of dysentery 

 observed in the kittens, and not the bacteria occur- 

 ring with the amoebae in the feces, was proven by 

 using pure cultures of the various bacteria from the 

 feces for feeding and injections, and mixed cultures 

 of all the bacteria that could be cultivated were also 

 used in the same manner, but in no case did diarrhoea 

 or dysentery result. While there were probably bac- 

 teria present which could not be cultivated, I do not 

 believe that this militates against the conclusion that 

 Entamceba histolytica was the cause of the lesions 

 produced. 



That the bacteria occurring in the feces of amoebic 

 dysentery cases are not the cause of the lesions ob- 

 served in experimental animals was previously proven 

 conclusively by Harris. He injected 4 dogs per 

 rectum with fecal material containing amoebae and 

 in all of them the injection was followed by dysentery. 

 In 2 cases abscess of the liver was found after death, 



