178 PARASITIC AMCEB^E OF MAN. 



In one of the infected kittens amoebic abscess of 

 the liver occurred and the pus from this abscess was 

 sterile except for the presence of a few amoebse, while 

 these parasites were also demonstrated in sections of 

 the abscess wall. This observation is almost conclusive 

 of the etiological relationship of Entamoeba Msto- 

 lytica to abscess of the liver complicating dysentery. 



Control experiments were also made with Enta- 

 moeba eoli with a negative result in every case. 



My experiments regarding the production of 

 dysentery in kittens by Entamoeba histolytica have 

 been confirmed by Werner, working at the Sailors' 

 Hospital in Hamburg. He experimented with two 

 strains of Entamoeba histolytica, only one of which 

 he found infective. He was able to produce dysentery 

 in cats with this strain, but found that after six 

 passages the organism lost its virulence. The incuba- 

 tion period varied from 4 to 13 days, the average 

 being 9 days. Of six cats infected with this species 

 four died, the duration of the disease varying from 

 7 to 24 days, the average being 15 days. The 

 animals were infected per rectum. Werner states 

 that the lesions were typical of amoebic dysentery and 

 were always confined to the colon, especially the 

 lower portion. Guinea-pigs and rats were found to 

 be resistant to infection with this parasite. 



From the evidence which has been submitted it 



