PARASITIC AMOEBJE OF MAN. 



arranged side by side. There is no visible karyosome, 

 but a few very minute granules of chromatin may 

 sometimes be observed in the hyaloplasm. In the fully 

 developed amoebse an oval body may be observed which 

 lies in contact with, or very near, the nucleus, and 

 which is about one-third the size of the latter. This 

 body undoubtedly corresponds to the cytoplasmic 

 body (Nebenkorper, centrosome, or blepharoplast ) 

 described by Schaudinn in Paramceba eilhardi. 



A nutritive vacuole is not present in this species, 

 so far as I have been able to determine, although 

 small oval bodies are sometimes present which sug- 

 gest vacuoles. 



In reproduction by simple division the cytoplasmic 

 body appears to divide first, followed quickly by the 

 division of the nucleus, and finally by the cytoplasm, 

 two daughter amoeba? being thus produced. 



In the amoebic stage the parasite stains poorly, 

 although it may be stained with Wright's method, 

 Heidenhain's iron hsematoxylin, carbol-fuchsin, Bor- 

 rel blue, or methylene blue. These stains do not dif- 

 ferentiate the ecto- and endoplasm, but the nucleus 

 stains fairly well and, when the organism is dividing, 

 shows well marked mitotic figures. With Wright's 

 stain the nucleus appears to be composed almost en- 

 tirely of chromatin, staining a pink or reddish violet, 

 while the cytoplasmic body may sometimes be dis- 



