X TABLE OF CONTENTS 



CHAPTER II. STERILIZATION, DISINFECTION, ANTISEPSIS, FOOD 

 PRESERVATION. 



Definitions, 62 ; Physical sterilization, 62 ; Mechanical removal, 62; Desiccation, 63; 

 Light, 63; Cold, 64; Heat, 64; Electricity, 71; Chemical sterilization, 71 ; Soaps, 

 71; Acids, 71; Alkalies, 73; Oxidizing agents, 73; Inorganic salts, 74; Organic 

 poisons, 76; Antiseptics and preservatives, 79; Physical, 79; Chemical, 79; 

 Testing of antiseptics and disinfectants, 80. 



CHAPTER III. CULTURE MEDIA. 



Definition, 83 ; Glass-ware, 83 ; The common media, 84 ; Nutrient broth, 84; Titra- 

 tion of media, 85; Gelatin, 88; Agar, 89; Modifications, 90; Sterilizable special 

 media, 91 ; Potato, 91; Milk, 92; Peptone solution, 92; Nitrate broth, 92; Blood- 

 serum, 92; Loeffler's blood-serum, 94; Eggs, 94; Dorset's egg, 94; Bread paste, 

 94; Media containing uncooked protein, 95; Sterile blood, 95; Ascitic fluid, 97; 

 Sterilization, 97; Sterile tissue, 98; Blood-streaked agar, 98; Blood-agar, 98; 

 Broth containing tissues, 99; Ascitic-fluid agar, 99; Ascitic fluid with tissue, 99. 



CHAPTER IV. COLLECTION OF MATERIAL FOR BACTERIOLOGICAL 



STUDY. 



General considerations, 100; Sampling water and foods, 100; Material from the 

 body, 100 ; Sputum, 101; Urine, 101; Blood and transudates, 101; Cerebro-spinal 

 fluid. 101; Feces and intestinal juice, 102; Pus and exudates, 102; Material from 

 autopsies, 103. 



CHAPTER V. THE CULTIVATION OF MICRO-ORGANISMS. 



Avoidance of contamination, 104; Isolation of bacteria, 105; Plate cultures, 106; 

 Roll tubes, no; Streak method, 112; Tall-tube method, 112; Colonies, 113; 

 Pure cultures, 113; Stock cultures, 114; Regulation of temperature, 115; High 

 temperature incubator, 115; Gas-regulator, 116; Automatic safety-burner, 120; 

 Incubator room, 120; Prevention of drying, 120; Low-temperature incubator, 

 121; Cultivation of anaerobic bacteria, 124; Deep stab, 124; Veillon tall-tube 

 method, 124; Fermentation tube, 125; Removal of oxygen, 125; Hydrogen at- 

 mosphere, 126; Further methods, 130. 



CHAPTER VI. METHODS OF ANIMAL EXPERIMENTATION. 



Value of Animal experimentation, 131 ; Care of animals, 131 ; Holding for operation, 

 132; Inoculation, 133; Subcutaneous and intraperitoneal, 133; Intracranial, 

 133; Into circulating blood, 133; Other sites, 134; Subcutaneous application, 

 134; Alimentary and respiratory infection, 134; Collodion capsules, 134; Obser- 

 vation of infected animals, 136. 



