CULTURE MEDIA 99 



coccus, but more especially for cultivation of the flagellated 

 hematozoa such as trypanosomes and related organisms, including 

 the Leishman-Donovan bodies. 



Smith's Broth Containing Sterile Tissue. Pieces of naturally 

 sterile organs, usually liver or kidney, are placed in broth, more 

 particularly in fermentation tubes of broth. The bits of tissue 

 are conveniently handled by touching with a hot platinum wire 

 or glass capillary, to which they will adhere. The medium is 

 especially useful for the culture of anaerobic bacteria. Naturally 

 sterile blood added to the broth also serves for this purpose. 



Ascitic-fluid-agar. This is made in the same way as the 

 Novy's blood-agar except that naturally sterile human ascitic 

 fluid is employed instead of blood. The medium is beautifully 

 transparent, and may be employed for plating as well as for tube 

 cultures. It is especially valuable for cultivation of the gono- 

 coccus and also for the streptococcus, pneumococcus and 

 meningococcus. 



Noguchi's 1 Ascitic Fluid with Sterile Tissue. Naturally 

 sterile tissue is placed in a tall tube. A deep layer of ascitic fluid 

 is added, and for some purposes this is covered with a layer of 

 sterile paraffin oil. The medium is used more especially for the 

 cultivation of the blood spirochetes which cause relapsing fever. 



1 Noguchi: Journ. Exp. Med., Jan. i, 1912, Vol. XV, pp. 90-100. 



