102 



BACTERIOLOGY 



Feces from infants and young children are best collected by 

 means of a heavy glass tube closed and rounded off at the end, and 

 provided with a lateral opening near the closed end. This is 

 enclosed in a larger tube and sterilized. It is inserted well into 

 the rectum with aseptic technic and the entrance of fecal material 

 through the lateral opening is favored by 

 gently moving the tube. It is then with- 

 drawn and replaced in its original container 

 to be transported to the laboratory. From 

 adults the feces are passed directly into a 

 sterilized covered agateware basin without 

 other special apparatus. 



Intestinal juice from the duodenum may 

 be obtained in infants 1 by inserting a sterile 

 rubber catheter, closed below with a steri- 

 lized gelatin capsule, through the esophagus 

 and stomach into the duodenum. The cap- 

 sule is then blown off by pressure from a 

 sterile syringe attached at the other end of 

 the catheter and the fluid contents of the 

 duodenum aspirated. In adults 2 the Einhorn 

 duodenal tube is employed. The tube is 

 of instrument for obtain- sterilized by boiling and the lower opening 



mg feces from infants for J 



bacteriological examina- sealed with a sterilized gelatin capsule and 

 by finally coating with shellac. The tube is 

 inserted through the esophagus and is carried 

 through the pylorus by peristalsis. Ordinarily it is inserted in 

 the evening. On the following morning the seal at the lower end 

 is broken by pressure of a sterile syringe attached to the free end 

 of the tube and the sample of juice aspirated. Intestinal juice 

 may be obtained from various levels in the jejunum also by regu- 

 lating the length of tube inserted. 



Pus and other exudates are best collected in sterile glass capil- 



FIG. 38. Two types 



tion. (After Schmidt and 

 Strasburger.) 



1 Hess: Journ. Infections Diseases, July 1912, Vol. XI, pp. 71-76- 

 2 MacNeal and Chace: Arch. Int. Med., Aug., 1913, Vol. XII, pp. 178-197- 



