SPIROCH^ET^E 



359 



anaerobic conditions. The technic of culture is somewhat diffi- 

 cult and the original papers should be consulted in detail. Inocu- 

 lation of the cultures into rabbits and monkeys has caused typical 

 syphilitic lesions. 



FIG. 143. Spirochceta pallida stained by Levaditi method. The section shows 

 an infarcted lymph vessel at the junction of two branches. The lumen is filled with 

 leukocytes. The spirochetes follow the lymph vessel for the most part, but are also 

 penetrating into the surrounding tissue. (From Doflein after Ehrmann.} 



Noguchi's luetin is prepared by grinding the solid medium 

 rich in spirochetes in a mortar and emulsifying it in a small 

 amount of fluid. This is then heated to 60 C. for an hour and 



