365 



Tube No. i, back row 



Tube No. 2, back row 



Complement i unit (o. 2 c.c.) 

 Patient's serum 0.08 c.c. 

 Salt solution 0.32 c.c. 



Complement i unit (0.2 c.c.) 

 Patient's serum o.oi c.c. 

 Sheep's corpuscles, i unit (o. 2 c.c.) 

 Salt solution 0.59 c.c. 



Mix thoroughly and incubate at 37 C. i hour. Then add: 



Mix thoroughly and incubate for i hour, recording the progress of hemolysis at. 

 intervals of 15 minutes. Then refrigerate 16 hours and record the final reading. 



Tube No. 3, front row 



Tube No. 4, front row 



Complement i unit (o. 2 c.c.) 

 Patient's serum 0.02 c.c. 

 Antigen i unit (o. i c.c.) 

 Salt solution o. 28 c.c. 



Complement i unit (0.2 c.c.) 

 Patient's serum o. 04 c.c. 

 Antigen i unit (o. i c.c.) 

 Salt solution o. 26 c.c. 



Mix thoroughly and incubate at 37 C. i hour. Then add: 



Sheep's corpuscles i unit } 



(0.2C.C.). 'o/LCC 1 



Hemolytic amboceptor 2 units ( 

 (0.2 c.c.). 



unit 



Sheep's corpuscles i 



(0.2 c.c.). I , 



Hemolytic amboceptor 2 units [ ' 



(0.2C.C.). 



Mix thoroughly and incubate for i hour, recording the progress of hemolysis at 

 intervals of 15 minutes. Then refrigerate 16 hours and record the final reading. 



1 The suspension of sheep's corpuscles containing i unit in o. 2 c.c. and the solu- 

 tion of hemolytic amboceptor containing 2 units in 0.2 c.c. are quickly mixed to- 

 gether in equal parts, and o . 4 c.c. of this homogeneous mixture is added at this point. 

 This procedure results in a saving of time as well as greater accuracy. 



