1102 THE BELL SYSTEM TECHNICAL JOURNAL, SEPTEMBER 1957 



included in the run. Once in the vial, the inoculum is shaken and added 

 to the aged sea water at the rate of 1 ml per 10 liters of medium. This 

 amount of inoculum was calculated to give the maximum number of 

 bacteria, consistent with a minimum addition of organic matter. The 

 carboy is then placed under slight, positive oxygen pressure. 



The test is run in 60 ml glass-stoppered bottles. A small amount of test 

 material is placed in each bottle. At the outset of the experiments, when 

 ground material was used, this amounted to 0.05 gram, or a surface area 

 of 4 to 45 sq cm, depending on the material. Later, when thin sheets of 

 about 4 mils thickness were used, the samples were cut to a size of 2.54 

 cm square. The samples are placed in the bottles the night before, and 

 enough aged sea water added to permit surface wetting. With many 

 materials this seems to result in less accumulation of air bubbles on the 

 surfaces of the materials during subsequent filling with the medium. 



Table III — Enrichment Cultures* Used as Supplementary 



Sources of Inoculum in Preparation of Enrichment 



Cultures for Current Program 



Ref. No. 



34-134 

 34-134 

 .34-134 

 34-134 

 34-134 

 34-132 



34-134 

 34-134 

 25-143 

 34-134 

 34-134 

 34-1.34 

 25-141 

 34-134 

 34-134 

 34-134 

 34-134 

 34-134 

 34-134 

 34-134 

 34-134 



Description 



rubber in distilled water 



anthracene in sea water 



sewage outfall, rubber in sea water 



mixed hydrocarbons in sea water 



garden soil, rubber in sea water 



Athabaska tar sand, hydrocarbon-oxidizing bacteria and 



sulfate-reducers in sea water 

 tricresol in sea water 

 mixed hydrocarbons in sea water 

 0.10% phenol in sea water 

 0.25% phenol in sea water 

 cork in sea water 

 Shell oil No. 10 in sea water 

 lignin in sea water 

 sewage outfall, rul)ber in sea water 

 sawdust and mud in sea water 

 garden soil, rubber in sea water 

 rubber in tap water 

 mi.xed crude oil in sea water 

 kerosene in sea water 

 paraffin in sea water 

 rubber in tap water 



Athabaska tar sand, mixed crude oil in sea water i; 



thiokol in sea water 

 neoprene in sea water 

 cellulose acetate in sea water 

 butadiene (Buna A) in sea water 



pooled aerobic hydrocarbon-o.xidizing bacteria in sea water 

 crude coal tar in sea water 

 shellac in sea water 



* Obtained from Dr. C. ZoBell, Scripps Institute of Oceanography. 



