23 



diseases (peritonitis and pleurisy). There are, therefore, but fifty- 

 four that should be counted. In them, there were twelve positive 

 results, or -28.57 per cent. (12 : 42) successes out of all the inocula- 

 tions. There were used thirty-two specimens from fourteen different 

 cows, and the successful results came from six different cows — that 

 is, 42.8 per cent. (G : 14) of the cows were shown in this way to have 

 infectious milk, and 37.5 per cent. (12:32) of the specimens used 

 were shown to have active infectious properties. 

 J^ositive results were obtained from 



Cow P (three times in two different inoculations). 



Cow D (three times in three different inoculations) . 



Cow F (ouce) . 



Slocum cow (once). 



Saunders cow (once). 



Mayhew cow (three times in two different inoculations). 

 The combining of the results obtained from both rabbits and 

 guinea-pigs shows the following : Successful results were obtained in 

 milk from cow P three times (two diff'ereut specimens) in guinea- 

 pigs, and twice in rabbits (two different specimens) ; from cow L 

 ouce in rabbits ; from cow O three times (three different specimens) 

 in guinea-pigs ; from cow F once in guinea-pigs ; from the Slocum 

 cow once in guinea-pigs ; from the Saunders cow once in guinea-pigs, 

 and twice in rabbits (two different specimens) ; and from the May- 

 hew cow three times in guinea-pigs (two different specimens) — that 

 is to say, out of fourteen cows used the milk was shown to be infec- 

 tious in seven, or 50 per cent, by inoculation experiments. 



An interesting fact is also shown, and that is, that bacilli were 

 found in the milk or cream, and successful inoculation experiments 

 made in animals with the same specimen in five different cases (in- 

 cluding eight of the successful ones') as follows : 



Compariso?i of the dates when Bacilli tvere found in the Milk and the same Milk 

 was used for successful inoculation experiments. 

 Cow. Positive. Positive. Positive. 



Cover-glass. Guinea pig. Rabbit. 



P- Cream, A. M. Cream, A. M., March 9, 1889 Cream, a. m., Marcii 9, 1889 



r',.oorv. D iv« \ Cream, P. M., March 9, 1889 Cream, P. m., March 9, 1889 



uream, p. m. j Cream, p. m., March 9, 1889 



O. 1st of milking, cream, Pirst of milking, cream, 

 March 9, 1889. March 9, 1889. 



Slocum. Last of milking. Last of milking. 



June 10, 1889 

 Mayhew. Last of milking, milk. Last of milking, milk, 

 June 21, 1889 



The inoculation experiments, above detailed, seem to me to be 

 deserving of consideration because they were done under the most 

 careful precautions that could be devised. In all cases the experi- 

 ment animals were kept under observation long enough to determine, 

 so far as could be seen, that they were in good health, and after the 

 inoculations they were separated and kept under close watch, but in 

 healthy surroundings. Some of those that were used were inoculated 

 immediately after purchase, because of a scarcity of the supply at 

 the farm, and were not in good condition. But as no sign of tuber- 



