CONTENTS 



PAGE 



INTRODUCTION > -,** . > . . . . . ;l 



PART I. CULTURE METHODS AND TECHNIQUE 



CHAI-TER 



I. ISOLATION AND PURE-CULTURE METHODS 9 



I. The Development and Application of Culture Methods . 10 



II. Cleaning Glassware .."' . . 12 



III. The Principles and Methods of Sterilization 15 



IV. Preparation of Culture Media 23 



Liquid Media .:,.-> . >. . . 23 



Solid Media : - .: . j, . . 26 



Neutralization of Culture Media . . . . .,;.,.. . 33 



V. Present Method of isolating Organisms .._... 34 



II. TECHNIQUE OF FIXING, IMBEDDING, AND STAINING ... 41 



I. Fixing . ' ; ; . . 41 



II. The Paraffin Process : Infiltration and Imbedding ... 45 



III. Staining . ... V : * ' ... 4^ 



PART II. PHYSIOLOGICAL RELATIONS 



III. GERMINATION STUDIES 55 



IV. GENERAL RELATIONS TO ENVIRONMENTAL FACTORS ... 62 



I. Saprophytism and Parasitism . ; .. . .-./. , ! . . . . 62 



II. General Relations to Climatological Factors 66 



III. Special Relations to Environmental Factors 69 



V. ARTIFICIAL INFECTION .... .?-. . V . . . ", . . 76 



VI. THE PRINCIPLES OF DISEASE CONTROL . . : , . . . . . 85 



I. Methods of Control *. .;?/,* 85 



II. Preparation of Fungicides . . . .'.,.. ,. . . 88 



PART III. FUNGOUS DISEASES OF PLANTS 



VII. GENERAL CLASSIFICATION. . . . . .r . .. ; . : . * . 93 



I. Fungous Diseases and Pathology 93 



II. The Classes of Fungi : . . . 94 



VIII. MYXOMYCETES. SLIME MOLDS 97 



I.'Phytomyxales (Phytomyxaceae) 97 



II. Club Root of Cabbage and Other Crucifers 97 



vii 



