MICRO ANALYTICAL AND BACTERIOLOGICAL LABORATORY 413 



A laboratory course of at least one hour each day extending through- 

 out the entire college year. The time necessary to do the laboratory 

 work will vary from day to day. The work is to be supplemented by 

 lectures, special readings and seminar work. The laboratory methods 

 employed are those of the Laboratory Section of the American Public 

 Health Association, The U. S. Public Health Service and the Bureau of 

 Chemistry of the U. S. Department of Agriculture, in-so-far as these 

 methods are applicable. 



I. Substances to be analyzed. 



1. Liquids of all kinds. 



2. Semiliquids and semisolids miscible with water. 



3. Solids of all kinds. 



II. Numerical and quantitative limits of comtamination in different substances. 



1. For mold quantity of spores and hyphae. 



2. For yeasts number and kind. 



3. For bacteria number and kind. 



4. For pus, dirt, sand, etc. 

 Ill Methods. 



1. Making concentrations. 



2. Making dilutions. 



3. Making the counts and estimates, 

 (a) Bacteria. 



(6) Yeasts. 



(c) Mold spores and mold hyphae. 



(d) Algae, in drinking waters, etc. 



(e) Protozoa. 



(/) Pus cells, in milk, etc. 

 (g) Dirt, sand, etc. 



4. Plate counts Petri dish cultures. 



(a) Culture media used. 



(b) Optimum temperature. 



(c) Time of incubation. 

 IV. Qualitative determinations. 



1. Apparatus. 



2. Culture media. 



3. Stains. 



4. Special methods. 



(a) Colon group of bacilli. 



(6) Presumptive colon bacillus test. 



(c) Sewage streptococci. 



(</) Dysentery bacilli and amoebae. 



(e) Bacillus typbosus. 



(/) Paratyphoid group. 



(g) Cholera vibrio. 



(h) Yeasts. 



(i) Molds. 



(i) Animal parasites. 



(k) Larvae, ovae, etc. 



