74 PHARMACEUTICAL BACTERIOLOGY. 



at another time, we now name the principal organisms which are Gram- 

 positive or Gram-negative. 



Bacteria and other Organisms Stained by the Gram Method. 

 Staphylococcus pyogenes aureus. 

 Staphylococcus pyogenes albus. 

 Streptococcus pyogenes. 

 Micrococcus tetragenus. 

 Micrococcus lanceolatus. 

 Bacillus diphtheriae. 

 Bacillus tuberculosis. 

 Bacillus 6f anthrax. 

 Bacillus of tetanus. 

 Bacillus of leprosy. 

 Bacillus aerogenes capsulatus. 

 Oidium albicans. 

 Actinomyces (of actinomycosis). 



Bacteria not Stained by the Gram Method 

 Diplococcus of meningitis (intracellular) . 

 Diplococcus of gonorrhea. 

 Micrococcus melitensis. 

 Bacillus of chancroids (Ducrey's). 

 Bacillus of dysentery (Shiga's). 

 Bacillus of typhoid fever. 

 Bacillus of bubonic plague. 

 Bacillus of influenza. 

 Bacillus coli communis. 

 Bacillus pyocyaneus. 

 Bacillus of Friedlander. 

 Bacillus proteus. 

 Bacillus pyocyaneus. 

 Bacillus mallei (glanders). 

 Spirillum of Asiatic cholera. 

 Spirillum of relapsing fever. 

 Bacillus of pneumonia. 



5. Pappenheim's Stain. 



Sat. Aqueous Sol. Methyl Green, 50 c.c. 



Sat. Aqueous Sol. Pyronine, 15 c.c. 



Mix and filter. This is much used for staining bacteria in pus and 

 other pathological secretions. The bacteria are stained a bright red, while 

 the cell nuclei are blue to purple. 



6. Smith's Stain. 



Stock Sol. Basic Fuchsin, 10 c.c. 

 Methyl Alcohol, 



Formaldehyde, each 10 c.c. 



Distilled Water, to make 100 c.c. 



